DEAE PuraBead HF


SKU: 3451 Category:


DEAE PuraBead® HF is a high performance weak anion exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of negatively charged bio-molecules.

DEAE PuraBead® HF ligand is di-ethyl-amino-ethyl (DEAE) coupled to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.

Please contact us for pricing for pack sizes of >1 litre.

Additional information

Weight N/A
Dimensions cm

Di-ethyl-amino-ethyl (DEAE)

Type of Ion Exchanger

Weak Anion

Absorbent appearance


Base Matrix

Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF)

Particle Size

90 ± 10µm

Total Ionic Capacity

110 – 160 μmol/g settled gel

Dynamic Binding Capacity

>45 mg BSA per mL at 300 cm/hr

Opertating Pressure

Up to 1 bar (15 psi)

Operational flow rate

Up to 600 cm/hr

Operating pH

pH2 to pH 14 (intermittent)

Chemical Stability

Stable in all commonly used buffers and solutions


0.5 to 1.0M NaOH, 25◦C


2-30◦C, 20% Ethanol