Description
DEAE PuraBead® HF is a high performance weak anion exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of negatively charged bio-molecules.
DEAE PuraBead® HF ligand is di-ethyl-amino-ethyl (DEAE) coupled to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.
Please contact us for pricing for pack sizes of >1 litre.
Additional information
Weight | N/A |
---|---|
Dimensions | cm |
Ligand | Di-ethyl-amino-ethyl (DEAE) |
Type of Ion Exchanger | Weak Anion |
Absorbent appearance | White |
Base Matrix | Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF) |
Particle Size | 90 ± 10µm |
Total Ionic Capacity | 110 – 160 μmol/g settled gel |
Dynamic Binding Capacity | >45 mg BSA per mL at 300 cm/hr |
Opertating Pressure | Up to 1 bar (15 psi) |
Operational flow rate | Up to 600 cm/hr |
Operating pH | pH2 to pH 14 (intermittent) |
Chemical Stability | Stable in all commonly used buffers and solutions |
Sanitization | 0.5 to 1.0M NaOH, 25◦C |
Storage | 2-30◦C, 20% Ethanol |