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Adeno-Associated Virus (AAV)

Adeno-associated virus (AAV) purification is a critical step in the production and application of AAV vectors, which have become essential tools in gene therapy and genetic research. AAVs are known for their safety, efficiency, and long-lasting gene expression, but the presence of impurities in AAV preparations can hinder their therapeutic and research potential. Purification processes, often involving chromatography and ultracentrifugation, help concentrate and isolate AAV particles while removing contaminants such as cellular debris, other viral particles, and host cell proteins. This purification ensures that AAV vector preparations are of high purity and potency, minimizing the risk of adverse immune responses and enhancing the precision of gene delivery. AAV purification is vital for developing safe and effective gene therapies and for conducting precise genetic studies, making it a cornerstone of the rapidly evolving field of gene-based medicine and genetic research.

Our Solutions

After primary capture, remove host cell contaminants using HCPure™.

Mixed mode polishing can increase sample purity while minimizing the risks of capsid loss.

  • Orthogonal mixed mode binding interactions remove host cell contaminants
  • Over 99% recovery of AAV2 capsids
  • Over 80% of host cell contamination removed

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Strong cation exchange resins for AAV capsid capture using SP PuraBead® P6HF.

  • Suitable for capture of wild, recombinant and mutant AAV serotypes
  • Caustic stable for high performance over multiple CIP cycles
  • Greater than 70% recovery of multiple CIP cycles
  • No ad5 co-elution

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Use strong anion exchange resins to enrich your AAV samples after capture and polishing.

 Q PuraBead® P6HF separates empty and full capsids, ensuring therapeutic potency and effectiveness.

  • Greater than 70% recovery of full AAV 8 capsids
  • Caustic stable for high performance over multiple CIP cycles
  • Flexible and scalable from benchtop to production scale

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