MiMode™ PuraBead® HX2
Product Overview
MiMode™ PuraBead® HX2 is a mixed-mode chromatography resin designed to provide a versatile and cost-effective solution for biomolecule purification. Built on the robust PuraBead® 6HF base matrix, it combines aromatic hydrophobic and hydrogen-bonding interactions, enabling both bind-and-elute and flow-through polishing applications.
Unlike single-mode affinity solutions, MiMode™ PuraBead® HX2 offers broad selectivity, making it suitable as a platform technology across multiple molecule classes. The synthetic ligand ensures strong base stability, while the PuraBead® 6HF matrix delivers excellent flow properties and scalability from discovery through to large-scale manufacturing.
With a wide operational pH range, compatibility with common buffer systems, and straightforward cleaning using sodium hydroxide, MiMode™ PuraBead® HX2 integrates seamlessly into existing workflows. It is available in high-throughput screening formats as well as process-scale volumes, supporting both rapid development studies and commercial production needs.
Key features of MiMode™ PuraBead® HX2
- Mixed-mode ligand combining hydrophobic and hydrogen bond interactions.
- Broad selectivity for capture and polishing across diverse biomolecule types.
- Operates under mild conditions, supporting product quality and stability.
- Robust base stability and tolerance to cleaning with 1.0 M NaOH.
- High-flow, low-pressure PuraBead® 6HF matrix ensures easy scalability.
Example Applications
MiMode™ PuraBead® HX2 has been successfully applied in a variety of contexts, including:
- Antibody purification – Capture and polishing of bispecifics, antibodies, as well as fragments and engineered formats.
- Protein polishing – Removal of aggregates, host cell proteins, and other process-related impurities in flow-through mode.
- General protein purification – Versatile capture and separation of recombinant proteins with diverse physicochemical properties.
Simplified workflow integration and scalability
MiMode™ PuraBead® HX2 can be used in screening, process development, and large-scale manufacturing. Available in 96-well plates, pre-packed columns, and bulk slurry, it is designed to support flexible adoption into diverse purification strategies. Combined with Astrea Bioseparations’ wider toolbox of resins and services, MiMode™ PuraBead® HX2 provides an adaptable option for streamlining downstream processing.
Specifications
Product specifications
| Feature | Details | Why it matters |
|---|---|---|
| Technology | Synthetic ligand-based adsorbent | Provides highly specific and efficient endotoxin binding while maintaining high protein recovery |
| Base matrix | PuraBead® | Ensures durability, high flow rates, and low non-specific binding |
| Mode of operation | Bind-and-elute chromatography | Fits into existing purification workflows |
| Binding capacity | 20 - 40 g/L (recombinant protein static binding capacity) | Effective binding of IgG |
| Column pre-packing | 1 mL to 30 L | Available pre-packed for flexible scalability, suitable for R&D, pilot studies, and large-scale manufacturing in a seamless plug-in format |
| Operating pH range | 1.5–14 (intermittent) | Works in a wide range of operating conditions, making it adaptable for different protein formulations |
| Optimal flow rate | Up to 500 cm/h | Ensures rapid processing without compromising binding efficiency |
| Regeneration & depyrogenation | 0.5 M–1.0 M NaOH | Enables reuse while maintaining high endotoxin clearance, reducing operational costs |
| Storage conditions | 2–30°C, 20% ethanol | Stored in preservative for stability and antimicrobial storage |
| Shelf life | 5 years | Reliable long-term performance for consistent results |
| Regulatory compliance | Designed for cGMP manufacturing | Suitable for regulatory-compliant biopharmaceutical production processes, complete with regulatory support file |
Frequently Asked Questions
Frequently asked questions
What is MiMode™ PuraBead® HX2?
MiMode™ PuraBead® HX2 is a high-performance chromatography adsorbent designed for efficient and cost-effective antibody binding region capture and purification.
What are the key features of MiMode™ PuraBead® HX2?
- Mixed-mode ligand combining hydrophobic and hydrogen bond interactions.
- Broad selectivity for capture and polishing across diverse biomolecule types.
- Operates under mild conditions, supporting product quality and stability.
- Robust base stability and tolerance to cleaning with 1.0 M NaOH.
- High-flow, low-pressure PuraBead® 6HF matrix ensures easy scalability.
- Demonstrates broad specificity for capture and purification of antibody binding regions, with affinity for both kappa and lambda chains. Comparable protein recovery to traditional Protein L for kappa light chain capture.
What formats is MiMode™ PuraBead® HX2 available in?
MiMode™ PuraBead® HX2 is available in 96-well plates, Robocolumns® and pre-packed columns ready to plug into your research or GMP process. It is also available in slurry format.
What is the recommended operational flow rate for MiMode™ PuraBead® HX2?
The recommended operational flow rate is up to 500 cm/hr, depending on column size.
What is the pH stability of MiMode™ PuraBead® HX2?
MiMode™ PuraBead® HX2 is stable in the long term (3 months) from pH 3 to pH 13. The recommended operating pH range for protein binding applications is pH 6.0 to pH 9.0, starting with a low pH elution step (5.0 – 3.0) with scope for further optimization.
How should MiMode™ PuraBead® HX2 be stored?
The recommended storage condition is 2 – 30°C in 20% ethanol.
How is MiMode™ PuraBead® HX2 regenerated?
Removal of any residual adsorbed material can be achieved by washing the column with 1.0 M NaOH. A contact time of 1 hour will normally suffice to ensure destruction of viable organisms, although up to 5 hours contact time may be required. No less than 5 column volumes are recommended. Once cleaning with NaOH is complete, wash with at least 3 bed volumes of equilibration buffer until the pH and conductivity of the column eluate is equal to that of the buffer entering the column. Complete this process prior to further use or storage in the storage buffer.
What types of biomolecules can MiMode™ PuraBead® HX2 be used with?
- Broad range of applications.
- Demonstrates broad specificity for capture and purification of antibody binding regions, with affinity for both kappa and lambda chains.
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