Description
Aminophenylboronate affinity adsorbents are used for the purification of a diverse range of macromolecules which possess 1,2-diols, 1,3-diols, 1,2-hydroxy acids and 1,2-hydroxylamine groups. These functionalities are present in glycoproteins, carbohydrates, nucleic acids (nucleosides, nucleotides and RNAs), and polyphenols (catechols, flavenoids). These adsorbents are particularly suited to the purification of glycoproteins, or the removal a glycoprotein and carbohydrate impurities from non-glycosylated molecules.
Please contact us for pricing for pack sizes of >1 litre.
Additional information
Weight | N/A |
---|---|
Dimensions | cm |
Ligand | m-Aminophenylboronic acid |
Absorbent appearance | White |
Base Matrix | Agarose 6XL (cross-linked 6% agarose) |
Particle Size | 90µm (45-165µm ≥95% of beads within this range) |
Binding Capacity | Glycoproteins – in the range of 10-20 g/L Carbohydrates – up to 30g/L |
Opertating Pressure | Up to 3 bar (45 psi) |
Operational flow rate | Up to 500 cm/hr with column diameter < 3cm Up to 200 cm/hr with column diameter > 5cm |
Operating pH | pH2 to pH 14 (intermittent) |
Chemical Stability | Stable in all commonly used buffers and solutions |
Sanitization | 0.5 to 1.0M NaOH, 25◦C |
Storage | 2-30◦C, 20% Ethanol |