Description
Aminophenylboronate affinity adsorbents are used for the purification of a diverse range of macromolecules which possess 1,2-diols, 1,3-diols, 1,2-hydroxy acids and 1,2-hydroxylamine groups. These functionalities are present in glycoproteins, carbohydrates, nucleic acids (nucleosides, nucleotides and RNA’s) and polyphenols (catechols, flavonoids). Aminophenylboronate Agarose affinity adsorbents are particularly suited to the purification of glycoproteins, or the removal of glycoprotein and carbohydrate impurities from non-glycosylated molecules.
Aminophenylboronate PuraBead™ 6XL is immobilised onto PBL’s PuraBead® base matrix, a highly cross-linked, near mono-dispersed agarose bead.
Please contact us for pricing for pack sizes of >1 litre.
Additional information
Weight | N/A |
---|---|
Dimensions | cm |
Ligand | m-Aminophenylboronic acid |
Absorbent appearance | White |
Base Matrix | Highly cross-linked 6% near monodisperse agarose (PuraBead 6XL) |
Particle Size | 100 ± 10µm |
Binding Capacity | Glycoproteins – in the range of 10-20 g/L Carbohydrates – up to 30g/L |
Opertating Pressure | Up to 3 bar (45 psi) |
Operational flow rate | Up to 500 cm/hr with column diameter < 3cm Up to 200 cm/hr with column diameter > 5cm |
Operating pH | pH2 to pH 14 (intermittent) |
Chemical Stability | Stable in all commonly used buffers and solutions |
Sanitization | 0.5 to 1.0M NaOH, 25◦C |
Storage | 2-30◦C, 20% Ethanol |