Description
CM PuraBead® HF is a high performance weak cation exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of positively charged bio-molecules. CM PuraBead® HF ligand is carboxy-methyl group (CM) to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.
Additional information
Weight | N/A |
---|---|
Dimensions | cm |
Ligand | Carboxymethyl (CM) |
Type of Ion Exchanger | Weak Cation |
Absorbent appearance | White |
Base Matrix | Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF) |
Particle Size | 90 ± 10µm |
Total Ionic Capacity | 90 to 130 μmol/g settled gel |
Opertating Pressure | Up to 1 bar (15 psi) |
Operational flow rate | Up to 600 cm/hr |
Operating pH | pH2 to pH 14 (intermittent) |
Chemical Stability | Stable in all commonly used buffers and solutions |
Sanitization | 0.5 to 1.0M NaOH, 25◦C |
Storage | 2-30◦C, 20% Ethanol |