CM PuraBead HF


SKU: 3450 Category:


CM PuraBead® HF is a high performance weak cation exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of positively charged bio-molecules. CM PuraBead® HF ligand is carboxy-methyl group (CM) to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.

Additional information

Weight N/A
Dimensions cm

Carboxymethyl (CM)

Type of Ion Exchanger

Weak Cation

Absorbent appearance


Base Matrix

Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF)

Particle Size

90 ± 10µm

Total Ionic Capacity

90 to 130 μmol/g settled gel

Opertating Pressure

Up to 1 bar (15 psi)

Operational flow rate

Up to 600 cm/hr

Operating pH

pH2 to pH 14 (intermittent)

Chemical Stability

Stable in all commonly used buffers and solutions


0.5 to 1.0M NaOH, 25◦C


2-30◦C, 20% Ethanol