LentiHERO®
Achieve highly efficient downstream purification of your lentiviral feedstocks and maximize the productivity of your lentiviral vector production system.
LentiHERO®’s large binding surface area is immediately accessible to lentiviral vectors even at very fast flow rates, ensuring capacity is high. Mild processing conditions ensure high recovery, protecting the yield and functionality of lentiviral vectors during downstream processing.
This product is covered by or for use under one or more patents (astreabioseparations.com/patents) and is provided subject to the terms of a Limited Use Label License Agreement (LULL). Accessible at: astreabioseparations.com/terms
| Name | SKU | Price | Quantity |
|---|---|---|---|
| LentiHERO® 1 | LH0001001A | $5,500.00 | |
| LentiHERO® 10 | LH0010001B | ||
| Nereus Spin Column | NL100100-new | $350.00 |
Product Details
Please Select Your ProductKey Features:
- High binding capacity of 4.7E+10 lentiviral particles per unit
- >60% recovery of lentiviral particles, tested in serum-free feedstocks
- 95% reduction in HCP contamination
- Lab-scale purification of lentiviral particles in 5 easy steps
- Mild process and elution condition
SKU:
NL100100-new
Product code:
NL100100-new
Binding chemistry:
Weak anion exchange
TYPICAL MODEL PROTEIN DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
recommended maximum capacity per device:
2E+10 viral particles
Options for loading:
Benchtop centrifuge
Vacuum manifold
Vacuum manifold
EQUIPMENT for elution:
Benchtop centrifugation
MAXIMUM VOLUME PER CENTRIFUGATION LOAD
BASED ON CENTRIFUGE TYPE:
BASED ON CENTRIFUGE TYPE:
Swing out : 15 mL
Fixed angle: 12 mL
Fixed angle: 12 mL
ADDITIONAL EQUIPMENT REQUIRED ER PURIFICATION:
50 mL centrifuge tubes with filter caps
RECOMMENDED OPERATIONAL centrifugation:
1000 x g
CHEMICAL STABILITY:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 0.5M NaOH, and solutions containing up to 10mM EDTA
CONNECTIONS:
Luer
INTENDED USE:
For research use only.
COMPLIANCE OF PERFORMANCE AND IDENTIFICATION:
Each product has passed a flow test and an inspection test.
Key Features:
- High binding capacity*
- Adherent feedstock, 4.5 E+11 viral particles/mL
- Suspension feedstock, 1.75 E+12 viral particles/mL
- Pseudotype independent recovery of functional particles**
- Adherent feedstock 2.6E+09 TU
- Suspension feedstock 1.9 E+09 TU
- Sanitizable with 0.5 M NaOH
*Example data, performance dependent on vector production variables
**Jurkat transduction assay
SKU:
LH0001001A
Product code:
LH0001001A
Binding Chemistry:
Weak anion exchange
TYPICAL DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
1mL
Nominal Void Volume:
3.5mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Luer
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone Functional membrane: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
Key Features:
- High binding capacity*
- Adherent feedstock, 4.5 E+11 viral particles/mL
- Suspension feedstock, 1.75 E+12 viral particles/mL
- Pseudotype independent recovery of functional particles**
- Adherent feedstock 2.6E+09 TU
- Suspension feedstock 1.9 E+09 TU
- Sanitizable with 0.5 M NaOH
*Example data, performance dependent on vector production variables
**Jurkat transduction assay
SKU:
LH0010001B
Product code:
LH0010001B
Binding Chemistry:
Weak anion exchange
TYPICAL DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
10mL
Nominal Void Volume:
30.0mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Sanitary flange (12.7mm)
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
Functional membrane: modified cellulose acetate
Functional membrane: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
Product Details
Key Features:
- High binding capacity of 4.7E+10 lentiviral particles per unit
- >60% recovery of lentiviral particles, tested in serum-free feedstocks
- 95% reduction in HCP contamination
- Lab-scale purification of lentiviral particles in 5 easy steps
- Mild process and elution condition
SKU:
NL100100-new
Product Specifications
Product code:
NL100100-new
Binding chemistry:
Weak anion exchange
TYPICAL MODEL PROTEIN DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
recommended maximum capacity per device:
2E+10 viral particles
Options for loading:
Benchtop centrifuge
Vacuum manifold
Vacuum manifold
EQUIPMENT for elution:
Benchtop centrifugation
MAXIMUM VOLUME PER CENTRIFUGATION LOAD
BASED ON CENTRIFUGE TYPE:
BASED ON CENTRIFUGE TYPE:
Swing out : 15 mL
Fixed angle: 12 mL
Fixed angle: 12 mL
ADDITIONAL EQUIPMENT REQUIRED ER PURIFICATION:
50 mL centrifuge tubes with filter caps
RECOMMENDED OPERATIONAL centrifugation:
1000 x g
CHEMICAL STABILITY:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 0.5M NaOH, and solutions containing up to 10mM EDTA
CONNECTIONS:
Luer
INTENDED USE:
For research use only.
COMPLIANCE OF PERFORMANCE AND IDENTIFICATION:
Each product has passed a flow test and an inspection test.
Product Details
Key Features:
- High binding capacity*
- Adherent feedstock, 4.5 E+11 viral particles/mL
- Suspension feedstock, 1.75 E+12 viral particles/mL
- Pseudotype independent recovery of functional particles**
- Adherent feedstock 2.6E+09 TU
- Suspension feedstock 1.9 E+09 TU
- Sanitizable with 0.5 M NaOH
*Example data, performance dependent on vector production variables
**Jurkat transduction assay
SKU:
LH0001001A
Product Specifications
Product code:
LH0001001A
Binding Chemistry:
Weak anion exchange
TYPICAL DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
1mL
Nominal Void Volume:
3.5mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Luer
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone Functional membrane: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
Product Details
Key Features:
- High binding capacity*
- Adherent feedstock, 4.5 E+11 viral particles/mL
- Suspension feedstock, 1.75 E+12 viral particles/mL
- Pseudotype independent recovery of functional particles**
- Adherent feedstock 2.6E+09 TU
- Suspension feedstock 1.9 E+09 TU
- Sanitizable with 0.5 M NaOH
*Example data, performance dependent on vector production variables
**Jurkat transduction assay
SKU:
LH0010001B
Product Specifications
Product code:
LH0010001B
Binding Chemistry:
Weak anion exchange
TYPICAL DYNAMIC BINDING CAPACITY
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
Bovine serum albumin at 1mg/mL in 10mM at Tris pH 8:
>60 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
10mL
Nominal Void Volume:
30.0mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Sanitary flange (12.7mm)
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
Functional membrane: modified cellulose acetate
Functional membrane: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
