pDNAHERO®
pDNAHERO® polish, a novel HIC adsorbent, offers an efficient orthogonal chromatography step for contaminant removal after AEX primary capture or post linearization.
The large binding surface area is highly accessible, providing consistent high binding capacity for pDNA at high flow rates. Performance is conserved as plasmid size increases, and delta pressure across the device remains low, avoiding pressure related sheer stress and loss of larger sequences.
Accomplish purification of large sequence plasmids at low operational pressure, without compromising on flow rate or yield, ensuring consistent yields.
This product is covered by or for use under one or more patents (astreabioseparations.com/patents) and is provided subject to the terms of a Limited Use Label License Agreement (LULL). Accessible at: astreabioseparations.com/terms
| Name | SKU | Price | Quantity |
|---|---|---|---|
| pDNAHERO® 1 polish | PHP0001001A | $5,500.00 | |
| pDNAHERO® 10 polish | PHP0010001B |
Product Details
Please Select Your ProductDescription:
Prepacked chromatography device designed for contaminant removal after AEX primary capture or post linearization at process development scale
Key Features:
- High dynamic binding capacity
- 4 mg/mL, (4.7 kb) pDNA sequence
- Low operational back pressure due to open unobstructive flow path
- Suitable for large plasmids (tested up to 34 kB)
- Sanitizable with 0.5 M NaOH
SKU:
PHP0001001A
Product code:
PHP0001001A
Binding Chemistry:
Hydrophobic interaction chromatography
TYPICAL DYNAMIC BINDING CAPACITY
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
>6 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
1 mL
Nominal Void Volume:
3.5 mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Luer
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
FUNCTIONAL MEMBRANE: modified cellulose acetate
FUNCTIONAL MEMBRANE: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
Description:
Prepacked chromatography device designed for contaminant removal after AEX primary capture or post linearization at process development scale
Key Features:
- High dynamic binding capacity
- 4 mg/mL, (4.7 kb) pDNA sequence
- Low operational back pressure due to open unobstructive flow path
- Suitable for large plasmids (tested up to 34 kB)
- Sanitizable with 0.5 M NaOH
SKU:
PHP0010001B
Product code:
PHP0010001B
Binding Chemistry:
Hydrophobic interaction chromatography
TYPICAL DYNAMIC BINDING CAPACITY
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
>6 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
10 mL
Nominal Void Volume:
30.0 mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Sanitary flange (12.7mm)
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
FUNCTIONAL MEMBRANE: modified cellulose acetate
FUNCTIONAL MEMBRANE: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
Product Details
Description:
Prepacked chromatography device designed for contaminant removal after AEX primary capture or post linearization at process development scale
Key Features:
- High dynamic binding capacity
- 4 mg/mL, (4.7 kb) pDNA sequence
- Low operational back pressure due to open unobstructive flow path
- Suitable for large plasmids (tested up to 34 kB)
- Sanitizable with 0.5 M NaOH
SKU:
PHP0001001A
Product Specifications
Product code:
PHP0001001A
Binding Chemistry:
Hydrophobic interaction chromatography
TYPICAL DYNAMIC BINDING CAPACITY
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
>6 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
1 mL
Nominal Void Volume:
3.5 mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Luer
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
FUNCTIONAL MEMBRANE: modified cellulose acetate
FUNCTIONAL MEMBRANE: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
Product Details
Description:
Prepacked chromatography device designed for contaminant removal after AEX primary capture or post linearization at process development scale
Key Features:
- High dynamic binding capacity
- 4 mg/mL, (4.7 kb) pDNA sequence
- Low operational back pressure due to open unobstructive flow path
- Suitable for large plasmids (tested up to 34 kB)
- Sanitizable with 0.5 M NaOH
SKU:
PHP0010001B
Product Specifications
Product code:
PHP0010001B
Binding Chemistry:
Hydrophobic interaction chromatography
TYPICAL DYNAMIC BINDING CAPACITY
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
Herring sperm DNA 0.05 mg/mL in 50mM at Tris, 5mM EDTA, 3M Ammonium sulphate, pH 8:
>6 mg/mL
Maximum Pressure at 20ºC:
6 Bar | 0.6 MPa | 87 psi
Bed Volume:
10 mL
Nominal Void Volume:
30.0 mL
Recommended Operational Flow Rate (MV/min):
5
Recommended Pre-Device Pressure:
3 Bar | 0.3 MPa | 43 psi
Chemical Stability:
Compatible with buffers commonly used in bioprocessing, including 20% EtOH, 1M acetic acid, 1M NaOH, and solutions containing up to 10mM EDTA
Connections:
Sanitary flange (12.7mm)
Vent Valve:
Screw cap
Repeated Use:
Single batch use
Materials of Construction:
HOUSING: Polypropylene, Silicone
FUNCTIONAL MEMBRANE: modified cellulose acetate
FUNCTIONAL MEMBRANE: modified cellulose acetate
Compliance of Materials:
All wetted parts have been assessed for low toxicity compliance and bio-compatibility against either USP VI <88>, ISO 10993, 21CFR Part 177 or by risk assessment
Declarations of Suitability:
TSE/BSE free, Non-Animal derived, Nitrosamine free statements available
Quality:
Manufactured within an ISO 9001:2015 Quality Management System the product is controlled, traceable and released through the Quality function. For research use only.
Compliance of Performance and Identification:
FUNCTIONAL MEMBRANE: dynamic binding capacity test/Lot membrane
DEVICES: Representative device samples tested by pressure flow and performance test
DEVICES: Representative device samples tested by pressure flow and performance test
