SP PuraBead HF


SKU: 3453 Category:


SP PuraBead® HF is a high performance strong cation exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of positively charged bio-molecules.

SP PuraBead® HF ligand is sulphonic acid group coupled via a propyl linker (SP) to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.

Please contact us for pricing for pack sizes of >1L.

Additional information

Weight N/A
Dimensions cm

Sulphonic acid with a propyl linker (SP)

Type of Ion Exchanger

Strong cation

Absorbent appearance


Base Matrix

Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF)

Particle Size

90 ± 10µm

Total Ionic Capacity

≥175 μmol/g settled gel

Binding Capacity

≥90 mg/mL of adsorbent (lysozyme)

Opertating Pressure

Up to 1 bar (15 psi)

Operational flow rate

Up to 600 cm/hr

Operating pH

pH2 to pH 14 (intermittent)

Chemical Stability

Stable in all commonly used buffers and solutions


0.5 to 1.0M NaOH, 25◦C


2-30°C, 20% ethanol/0.2 M sodium acetate (preservative)