Description
SP PuraBead® HF is a high performance strong cation exchange chromatography adsorbent designed for capture, intermediate or polishing steps for the purification of positively charged bio-molecules.
SP PuraBead® HF ligand is sulphonic acid group coupled via a propyl linker (SP) to highly cross-linked monodisperse 6% agarose (PuraBead® HF) which has excellent flow properties. The adsorbent is stable in up to 1.0M sodium hydroxide which allows for stringent cleaning and sanitization protocols.
Please contact us for pricing for pack sizes of >1L.
Additional information
Weight | N/A |
---|---|
Dimensions | cm |
Ligand | Sulphonic acid with a propyl linker (SP) |
Type of Ion Exchanger | Strong cation |
Absorbent appearance | White |
Base Matrix | Highly cross-linked 6% near monodisperse agarose (PuraBead 6HF) |
Particle Size | 90 ± 10µm |
Total Ionic Capacity | ≥175 μmol/g settled gel |
Binding Capacity | ≥90 mg/mL of adsorbent (lysozyme) |
Opertating Pressure | Up to 1 bar (15 psi) |
Operational flow rate | Up to 600 cm/hr |
Operating pH | pH2 to pH 14 (intermittent) |
Chemical Stability | Stable in all commonly used buffers and solutions |
Sanitization | 0.5 to 1.0M NaOH, 25◦C |
Storage | 2-30°C, 20% ethanol/0.2 M sodium acetate (preservative) |