MiMode™ PuraBead® CBX1

Product overview

MiMode™ PuraBead® CBX1 is a mixed-mode chromatography resin functionalized with an aminophenylboronic acid ligand. This chemistry enables a unique combination of cationic, aromatic hydrophobic, boronate ester, and hydrogen-bonding interactions, providing versatile selectivity for biomolecule purification. Built on the robust PuraBead® 6XL matrix, CBX1 offers high binding capacity, excellent flow properties, and strong base stability, making it well-suited for both bind-and-elute capture and flow-through polishing applications.

CBX1 is particularly effective in workflows requiring glycoprotein handling, including the selective enrichment of glycosylated antibodies and enzymes, removal of glycoprotein impurities from recombinant proteins, and DNA polishing in viral vector production. Its reversible boronate–diol complexation supports efficient capture and mild elution, ensuring preservation of product integrity while delivering high yields. With broad operating pH tolerance and compatibility with standard cleaning protocols, CBX1 integrates seamlessly into existing downstream processes from discovery through to manufacturing scale.

Key features of MiMode™ PuraBead® CBX1

• Mixed-mode ligand offering cationic, hydrophobic, boronate ester, and hydrogen-bonding interactions.
• Specific binding to cis-diol groups of glycosylated biomolecules.
• Robust PuraBead® 6XL base matrix supports high flow and scalability.
• Strong base stability and tolerance to cleaning with 0.5–1.0 M NaOH.
• Operates across a wide pH range  (pH 2–14 intermittent, 3–12 long term).
• High binding capacity for glycoproteins (10–20 g/L) with mild elution using sorbitol or low pH buffers.

Example Applications

MiMode™ PuraBead® CBX1 has demonstrated strong performance in:

• Antibody purification – Selective enrichment of glycosylated IgG from plasma, preserving structure and functionality.
• Recombinant protein polishing – Removal of glycoprotein impurities from yeast-expressed proteins to improve product purity and regulatory compliance.
• Lentiviral vector workflows – Effective in DNA polishing and impurity reduction for advanced therapy applications.
• General glycoprotein separations – Capture and purification of enzymes, glycoproteins, and glycated proteins for therapeutic, diagnostic, and analytical uses.

Simplified workflow integration and scalability

MiMode™ PuraBead® CBX1 is available in multiple formats including 96-well plates, RoboColumns®, 1 mL and 5 mL pre-packed columns, and bulk slurry, enabling straightforward progression from high-throughput screening to process development and GMP manufacturing. Its strong performance across scales, coupled with Astrea Bioseparations’ wider portfolio of resins and column technologies, makes CBX1 a flexible and cost-effective solution for modern downstream purification strategies.