MiMode™ PuraBead® AX1

Product overview

MiMode™ PuraBead® AX1 is an anion-exchange mixed-mode chromatography resin combining ionic and hydrophobic interactions. This dual binding mechanism provides enhanced impurity clearance compared to conventional anion exchangers, delivering selectivity for negatively charged microenvironments in target biomolecules such as nucleic acids, viral particles, and acidic proteins. Built on the robust PuraBead® base matrix, AX1 maintains strong flow performance and chemical stability across a wide pH and conductivity range, making it suitable for both capture and polishing operations.

By balancing strong binding with controlled elution conditions, AX1 enables high-resolution separation and reduces the need for additional downstream steps. Its mixed-mode chemistry ensures robust performance across diverse feed streams, while compatibility with standard cleaning protocols supports seamless integration into existing workflows.

Key features of MiMode™ PuraBead® AX1

• Combines anion-exchange and hydrophobic interactions.
• Operates effectively across a wide range of pH and salt conditions.
• Robust agarose base matrix with strong chemical stability.
• Reusable and compatible with 0.5–1.0 M NaOH cleaning.

Simplified workflow integration and scalability

AX1 is available in 96-well plates, RoboColumns®, pre-packed columns, and bulk slurry, supporting rapid screening, method development, and process-scale implementation. Its target-dependent performance highlights the importance of screening, allowing developers to identify the most effective conditions for their specific process.