MiMode™ PuraBead® HL3

MiMode™ PuraBead® HL3 is a mixed-mode chromatography resin designed to provide a versatile and cost-effective solution for biomolecule purification. Built on the robust PuraBead® 6HF base matrix, it combines aromatic hydrophobic and hydrogen-bonding interactions, enabling both bind-and-elute and flow-through polishing applications.

Unlike single-mode affinity solutions, MiMode™ PuraBead® HL3 offers broad selectivity, making it suitable as a platform technology across multiple molecule classes. The synthetic ligand ensures strong base stability, while the PuraBead® 6HF matrix delivers excellent flow properties and scalability from discovery through to large-scale manufacturing.

With a wide operational pH range, compatibility with common buffer systems, and straightforward cleaning using sodium hydroxide, MiMode™ PuraBead® HL3 integrates seamlessly into existing workflows. It is available in high-throughput screening formats as well as process-scale volumes, supporting both rapid development studies and commercial production needs.

Key features of MiMode™ PuraBead® HL3

• Mixed-mode ligand combining hydrophobic and hydrogen bond interactions.
• Broad selectivity for capture and polishing across diverse biomolecule types.
• Operates under mild conditions, supporting product quality and stability.
• Robust base stability and tolerance to cleaning with 1.0 M NaOH.
• High-flow, low-pressure PuraBead® 6HF matrix ensures easy scalability.

Example Applications

MiMode™ PuraBead® HL3 has been successfully applied in a variety of contexts, including:

• Protein purification – Versatile separation of recombinant proteins and with diverse physicochemical properties.
• Antibody purification – Capture and polishing of recombinant and plasma-based antibodies. Optimised for IVIG purification from human plasma and human IgG subtypes ((IgG1, IgG2, IgG3 & IgG4)

Simplified workflow integration and scalability

MiMode™ PuraBead® HL3 can be used in screening, process development, and large-scale manufacturing. Available in 96-well plates, pre-packed columns, and bulk slurry, it is designed to support flexible adoption into diverse purification strategies. Combined with Astrea Bioseparations’ wider toolbox of resins and services, MiMode™ PuraBead® HL3 provides an adaptable option for streamlining downstream processing.