Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product caprture and polishing. However, significant optimisation of binding and elution conditions is often required to achieve the desired level of purity.
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A scale-down reuse study was performed on an automated chromatography workstation using a 2 mL IsoClear B™ column challenged with 50 CV of plasma per cycle.
At Astrea Bioseparations we put product quality first and have committed to become the first chromatography adsorbent manufacturer to achieve full GMP compliance!
This article reviews the past and current options for screening of chromatography adsorbents for downstream process development and the use of emergent mixed mode ligand adsorbents for improved adsorb
Astrea Bioseparations has been manufacturing chromatography adsorbents on the Isle of Man since 1989. In this article we will update you on the expansion of our adsorbent manufacturing capacity.
Our goal is to provide you with a fully validated bespoke chromatography adsorbent and all the associated process conditions for purification of your target biomolecule.
In response to demand we have developed an affinity adsorbent for the capture and purification of antibody fragments.
We offer an extensive range of off-the-shelf Bioseparation products for the recovery and purification of biologicals as well as purification services including bespoke custom designed chromatography.
User friendly and ergonomic; performance is comparable to more expensive columns and the single use aspect compares well to lengthy cleaning cycles.
EtoxiClear™ has been shown to provide >3.4 log clearance of endotoxin from Fab fragments from an E. coli cell lysate, as well as from proteins such as IgG and HSA.
A number of methods have been adopted for the removal of endotoxin based on adsorption, in particular ion-exchange chromatography.
In this application note we describe how human serum albumin is captured and purified from human source plasma using Mimetic Blue® SA HL P6XL.
AlbuPure® can be used to capture and purify recombinant albumin-fusion proteins from cell culture supernatants with high purity and recovery.
Endotoxin or lipopolysaccharides (LPS) are highly toxic components of the cell wall of Gram-negative bacteria and are often present in significant amounts in bacterial cell expression systems.
