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Technical User Guides
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Technical User Guides
MAbsorbent® A2P HF & A2P HF LL Technical User Guide
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Version 3
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MiMode PuraBead® HL3
MiMode PuraBead® HL3
MiMode PuraBead® HL3
MiMode PuraBead® HL3 Alternative Image
MiMode PuraBead® HL3 Alternative Image
MiMode PuraBead® HL3 Alternative Image

MiMode PuraBead® HL3

From £400.00 Excl. VAT
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MiMode PuraBead® HL3 is one of four resins in the hydrophobic mixed-mode range that harnesses both hydrophobic and hydrogen-bonding interactions to target proteins and viral particles where other approaches often fail. The hydrophobic family includes MiMode PuraBead® HX1, HX2, HL3, and HL4 resins, each tuned to offer slightly different binding profiles, allowing developers to identify the optimal solution for their molecule.
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Product overview

MiMode PuraBead® HL3 is a mixed-mode chromatography resin designed to provide a versatile and cost-effective solution for biomolecule purification. Built on the robust PuraBead® 6HF base matrix, it combines aromatic hydrophobic and hydrogen-bonding interactions, enabling both bind-and-elute and flow-through polishing applications.

Unlike single-mode affinity solutions, MiMode PuraBead® HL3 offers broad selectivity, making it suitable as a platform technology across multiple molecule classes. The synthetic ligand ensures strong base stability, while the PuraBead® 6HF matrix delivers excellent flow properties and scalability from discovery through to large-scale manufacturing.

With a wide operational pH range, compatibility with common buffer systems, and straightforward cleaning using sodium hydroxide, MiMode PuraBead® HL3 integrates seamlessly into existing workflows. It is available in high-throughput screening formats as well as process-scale volumes, supporting both rapid development studies and commercial production needs.

Key features of MiMode PuraBead® HL3

  • Mixed-mode ligand combining hydrophobic and hydrogen bond interactions
  • Broad selectivity for capture and polishing across diverse biomolecule types
  • Operates under mild conditions, supporting product quality and stability
  • Robust base stability and tolerance to cleaning with 1.0 M NaOH
  • High-flow, low-pressure PuraBead® 6HF matrix ensures easy scalability

Example applications

MiMode PuraBead® HL3 has been successfully applied in a variety of contexts, including:

  • Protein purification: Versatile separation of recombinant proteins and with diverse physicochemical properties
  • Antibody purification: Capture and polishing of recombinant and plasma-based antibodies. Optimised for IVIG purification from human plasma and human IgG subtypes (IgG1, IgG2, IgG3, & IgG4)

 

Simplified workflow integration and scalability

MiMode PuraBead® HL3 can be used in screening, process development, and large-scale manufacturing. Available in 96-well plates, pre-packed columns, and bulk slurry, it is designed to support flexible adoption into diverse purification strategies. Combined with Astrea Bioseparations’ wider toolbox of resins and services, MiMode PuraBead® HL3 provides an adaptable option for streamlining downstream processing.

 

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