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Mix it up with MiMode™

The next generation of mixed-mode chromatography resins

MiMode™ PuraBead® mixed-mode resins deliver advanced selectivity and robust performance for purification of challenging biologics. Designed to combine multiple binding interactions, the MiMode™ range enhances impurity separation, maximizes yield, and simplifies downstream processing. With scalable formats and broad buffer compatibility, MiMode™ PuraBead® enables flexible, high-performance solutions from early development to manufacturing.

Introducing the newest hydrophobic mixed-mode family, MiMode™ PuraBead® HX1, HX2, HL3, and HL4 resins, built to take on the toughest separations. The MiMode™ PuraBead® hydrophobic mixed-mode range harness hydrophobic and hydrogen-bonding mixed-mode interactions for the purification of tricky proteins and viruses where other approaches fall short.

In action: Seamlessly screen the hydrophobic mixed-mode range by using our newest available format: MiMode™ PuraBead® Mixed-H 96-well plates — for high-throughput screening in a simple, smart, and effective way.

The MiMode™ range brings mixed-mode power to your toughest separations — flexible tools, smarter workflows, better results.

Discover why MiMode™ matters and how Astrea Bioseparations' history has paved the way for today’s breakthroughs. Explore the full story in our latest blog:

Mixed messages Bispecific capture

Choose your chemistry

Choosing the right chemistry is guided by the nature of your target molecule and its physicochemical properties, such as charge, hydrophobicity, and structural complexity. Each MiMode™ option is designed to exploit these molecular characteristics, providing unique selectivity for a wide range of biomolecules. If you are uncertain which mode will deliver the best performance, we recommend beginning with a broad screen across all MiMode™ chemistries. This approach allows you to quickly identify optimal separation conditions, streamline your purification strategy, and gain confidence that no potential solution has been overlooked.

Explore MiMode

Explore MiMode™

Hydrophobic, anion exchange, or cation exchange—there’s a mode for every molecule! Learn more with our brochure.

Hydrophobic

Hydrophobic

Target the tough ones. Hydrophobic and hydrogen bonding mixed-mode interactions for unique selectivity for tough proteins, viral vectors, and aggregates.

Anion exchange

Anion exchange

Versatile and precise. Anion exchange and hydrophobic mixed-mode interactions for nucleic acid clearance, viral purification, and negatively charged microenvironments.

Cation exchange

Cation exchange

Powerful capture, smart selectivity. Molecular microenvironments interact with unique cation, hydrophobic, and hydrogen bonding modes.

Explore by application

In addition to chemistry-based selection, you can also browse our solutions by application area as a guide to find the right tools for your specific purification challenge. Our team is continually uncovering new and exciting applications for our product lines. If you have a specific area of interest, we can share tailored technical guidance and the latest insights to support your work.

Recombinant proteins

Antibodies Bispecifics & fragments Erythropoietin & Cytokines Enzyme replacement therapies (ERTs)

Plasma-derived proteins

Serum albumin Clotting factors Immunoglobulins Glycosylated hemoglobin

Polishing

Host cell impurities Aggregates

MiMode™ PuraBead Naming Guide

Each product name (e.g. HX1, CX2, AX1) encodes details about its chemistry. Expand below to see how the names break down.

📖 Naming guide
MiMode™ Product NameUnique interaction typeOther interaction typesLigand coreLigand variantLigand density variant
HX1HydrophobicHydrogen bondingTriazineVariant 1Mid-density variant
HX2HydrophobicHydrogen bondingTriazineVariant 2Mid-density variant
HL3HydrophobicHydrogen bondingTriazineVariant 3Low-density variant
HL4HydrophobicHydrogen bondingTriazineVariant 4Low-density variant
CX1Cation exchangeHydrophobic & hydrogen bondingTriazineVariant 5Mid-density variant
CX2Cation exchangeHydrophobic & hydrogen bondingTriazineVariant 6Mid-density variant
CBX1Cation exchangeBoronate ester, hydrophobic & hydrogen bondingTriazineVariant 7Mid-density variant
CBX2Cation exchangeBoronate ester, hydrophobic & hydrogen bondingTriazineVariant 7Mid-density variant
AX1Anion exchangeHydrophobicNon-triazineVariant 8Mid-density variant

Perform with power

Optimize your purification strategy for maximum yield, purity, and process efficiency, no matter your target molecule. Whether you are developing early-stage concepts or refining large-scale manufacturing processes, MiMode™ resins are designed to adapt and perform where it matters most. With broad buffer compatibility and robust dual-mode selectivity, they provide the flexibility needed to tackle even the most complex feed streams.

Our scientists are ready to guide you through every stage of development — from small-scale screening to full cGMP manufacturing — helping you unlock the full potential of MiMode™ resins and accelerate your path to reliable, scalable purification.

Application notes

Explore real-world workflows using MiMode™ resins in diverse separations, including antibody capture, plasma protein purification, lentiviral vector polishing, and impurity clearance. These examples highlight how MiMode™ can streamline process development, improve yields, and reduce impurities across challenging bioprocesses.

Explore the full range of our most up-to-date application data

Speak to an expert

Scale seamlessly

Once optimized at small scale, seamlessly transition to larger volumes with our range of robust process columns:

  • SNAP®
  • Evolve®R
  • Evolve®
  • Evolve®D
  • Evolve® process columns

Unique selectivity, broad compatibility

MiMode™ mixed-mode resins offer unique selectivity, enabling efficient resolution of complex feed streams. Designed for compatibility with multiple biomolecule classes, these resins provide flexibility across diverse platform processes. Their dual-mode interaction mechanism enhances binding capacity while maintaining high resolution and recovery.

Consistency you can trust

Manufactured with precision and reproducibility, MiMode™ resins ensure consistent performance at every stage of development. Scale confidently with a proven technology that supports both early discovery and full cGMP-manufacturing environments. Our chromatography solutions deliver reliable results under demanding conditions.

Expert support, smoother scale-up

With expert technical support, Astrea Bioseparations helps you accelerate process development and achieve smooth, predictable scale-up.

Interested to learn more about our columns and column packing services?

 

Contact us

 
MiMode™ PuraBead® HX1
MiMode™ PuraBead® HX1
MiMode™ PuraBead® HX1 is one of four resins in the hydrophobic mixed-mode range that harnesses both hydrophobic and hydrogen-bonding interactions to target proteins and viral particles where other approaches often fail. The hydrophobic family includesMiMode™ PuraBead® HX1, HX2, HL3, and HL4 resins, each tuned to offer slightly different binding profiles, allowing developers to identify the optimal solution for their molecule.
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MiMode™ PuraBead® HX2
MiMode™ PuraBead® HX2
MiMode™ PuraBead® HX2 is one of four resins in the hydrophobic mixed-mode range that harnesses both hydrophobic and hydrogen-bonding interactions to target proteins and viral particles where other approaches often fail. The hydrophobic family includes MiMode™ PuraBead® HX1, HX2, HL3, and HL4 resins, each tuned to offer slightly different binding profiles, allowing developers to identify the optimal solution for their molecule.
View Details
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL3
MiMode™ PuraBead® HL3 is one of four resins in the hydrophobic mixed-mode range that harnesses both hydrophobic and hydrogen-bonding interactions to target proteins and viral particles where other approaches often fail. The hydrophobic family includes MiMode™ PuraBead® HX1, HX2, HL3, and HL4 resins, each tuned to offer slightly different binding profiles, allowing developers to identify the optimal solution for their molecule.
View Details
MiMode™ PuraBead® AX1
MiMode™ PuraBead® AX1
MiMode™ PuraBead® AX1 is an anion exchange mixed-mode family, harnessing anionic and hydrophobic to provide strong selectivity for negatively charged biomolecules. These resins excel in applications such as nucleic acid clearance, viral purification, and polishing of therapeutic proteins. The mixed-mode design ensures a balance between binding strength and elution control, even in the presence of complex feed streams or high salt environments.
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MiMode™ PuraBead® CBX1
MiMode™ PuraBead® CBX1
MiMode™ PuraBead® CBX1 is part of the cation exchange mixed-mode family, harnessing cationic, hydrophobic, boronate ester and hydrogen-bonding interactions for powerful purification of positively charged biomolecules, including monoclonal antibodies, enzymes, and proteins with isoelectric points above neutral. By combining multiple binding interaction contributions, these resins provide enhanced flexibility in binding and release, supporting both capture and polishing steps in bioprocess workflows.
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200 µm RoboColumns
200 µL RoboColumns®
Highly efficient miniaturized chromatography columns designed for rapid process development and high-throughput screening. Ready-to-use and pre-packed with a variety of resins, these columns are perfectly suited for automated liquid handling systems, ensuring convenience and precision.
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Resin Screening Kit 4 x 1mL
Resin Screening Kit 4 x 1mL
RSK4x1mL
Choose your flavour! Four high-throughput pre-packed columns, each packed with 1 mL of resin of your choice. Ideal for screening and small scale experiments in downstream process development. The convenient pre-packed format is perfectly suited for screening, scouting, and process optimization in both automated and manual settings.
Build Your Kit
Evolve® Process Columns
Evolve® Process Columns
Evolve® Process Columns have been designed specifically for use in biopharmaceutical applications. Available in eight diameters, Evolve® Process Columns provide cost effective, scalable, high performance technology suitable for use in both pilot and production scale chromatographic processes.
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Evolve®D Pre-packed Columns
Evolve®D Pre-packed Columns
Evolve D Pre-packed Columns offer a ready-to-use, versatile solution for bioprocessing and biomanufacturing. With a range of sizes and materials, these columns ensure efficiency and quality in protein purification and contaminant removal.
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